ABSTRACT
PURPOSE: Adiponectin is expressed in adipose tissue, and is affected by smoking, obesity, and genetic factors, such as CDH13 polymorphism, contributing to the development of coronary vascular diseases (CVDs). MATERIALS AND METHODS: We investigated the effect of genetic variations of CDH13 (rs3865188) on blood chemistry and adiponectin levels in 345 CVD patients undergoing statin-free or statin treatment. RESULTS: Genetic variation in CDH13 was significantly correlated with several clinical factors, including adiponectin, diastolic blood pressure, triglyceride (TG), and insulin levels. Subjects with the T allele (mutant form) had significantly lower adiponectin levels than those with the A allele. Total cholesterol (TC), low-density lipoprotein cholesterol (LDLc), TG/high-density lipoprotein cho-lesterol (HDLc) ratio, and HDL3b subtype were markedly decreased in statin treated subjects regardless of having the A or T allele. TG and TG/HDL in the statin-free group with TT genotype of the rs3865188 was higher than in the others but they were not different in the statin-treated subjects. We observed a significant difference in adiponectin levels between patients with the A and T alleles in the statin-free group; meanwhile, no difference in adiponectin levels was noted in the statin group. Plasma levels of other cytokines, leptin, visfatin, interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha), were not different among the CDH13 genotypes according to statin administration. Body mass index (BMI), TG, insulin, HDL3b, and TG/HDL ratio showed negative correlations with adiponectin levels. CONCLUSION: Plasma adiponectin levels and TG/HDL ratio were significantly different according to variants of CDH13 and statin administration in Korean patients with CVD.
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Adiponectin/blood , Alleles , Blood Pressure/genetics , Body Mass Index , Cadherins/blood , Cholesterol , Cholesterol, LDL , Genotype , Hydroxymethylglutaryl-CoA Reductase Inhibitors/therapeutic use , Insulin , Interleukin-6 , Leptin/genetics , Lipoproteins, HDL/genetics , Obesity/blood , Polymorphism, Genetic , Triglycerides/genetics , Tumor Necrosis Factor-alpha/genetics , Vascular Diseases/drug therapyABSTRACT
Apolipoprotein CIII (apo-CIII) participates in the regulation of triglyceride-rich lipoprotein metabolism. Several polymorphic sites have been detected within and around the apo-CIII gene. Here, we examined the relationship between apo-CIII SstI polymorphism (CC, CG, GG genotypes) and plasma triglyceride (TG) levels in a group of 159 Japanese individuals living in Southern Brazil. The sample was divided into a group of Japanese descendants (N = 51) with high TG (HTG; >200 mg/dL) and a group of Japanese descendants (N = 108) with normal TG (NTG; <200 mg/dL). TG and total cholesterol levels were analyzed by an enzymatic method using the Labtest-Diagnostic kit and high- and low-density lipoproteins by a direct method using the Labtest-Diagnostic kit and DiaSys Diagnostic System International kit, respectively. A 428-bp sequence of apo-CIII gene was amplified using oligonucleotide primers 5' GGT GAC CGA TGG CTT CAG TTC CCT GA 3' and 5' CAG AAG GTG GAT AGA GCG CTG GCC T 3'. The PCR products were digested with a restriction endonuclease SstI. Rare G allele was highly prevalent in our study population (0.416) compared to Caucasians (0.00-0.11). G allele was almost two times more prevalent in the HTG group compared to the NTG group (P < 0.001). The genotype distribution was consistent with the Hardy-Weinberg equilibrium. There was a significant association between rare G allele and HTG in Japanese individuals living in Southern Brazil as indicated by one-way ANOVA, P < 0.05.
Subject(s)
Aged , Female , Humans , Male , Middle Aged , Apolipoprotein C-III/genetics , Polymorphism, Genetic/genetics , Triglycerides/genetics , Alleles , Asian People/genetics , Brazil , Coronary Artery Disease/ethnology , Coronary Artery Disease/genetics , Gene Frequency , Genotype , Japan/ethnology , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , Triglycerides/bloodABSTRACT
Se midieron las concentraciones séricas de colesterol y triglicéridos en 152 adolescentes (53 varones y 99 mujeres) que acudieron a control de salud al Consultorio Integral de Adolescentes Valparaíso. Las concentraciones de colesterol total fluctuaron entre 120 mg/dl (percentil 10) y 220 mg/dl (percentil 90) y 20,4%estaban sobre 200 mg/dl. Las de triglicéridos fluctuaron entre 57 mg/dl (percentil 10) y 161 mg/dl (percentil 90) siendo 11,9%mayores que 150 mg/dl. Sólo se encontró una tendencia a la asociación entre concentraciones de colesterol total y desarrollo mamario, en cambio se encontró asociación clara entre colesterol e índice de masa corporal (p < 0,01) y antecedente familiar de enfermedad coronaria (p < 0,02)